Phenotypic strength of hecate alleles.

Embryos from females homozygous for hec mutant alleles were scored at 24 hpf for ventralization phenotypes according to [24]. Results were pooled from clutches from 15 different females for each allele.1)Severe radial ventralization and lack of all dorsoanterior structures.2)Absence of anterior structures, including posterior head structures such as the otoliths, lack of notochord, and expanded posterior somites.3)Absence of anterior head structures including the eyes, lack of notochord, and expanded posterior somites.4)Reduced anterior head structures such as the eyes but otherwise normal.5)In weak mutant clutches, a small fraction of embryos exhibit a duplicated axes, each of which often exhibits deletions of the anterior-most structures.6)Clutches from wild-type female can produce rare ventralized embryos.