AggreBots: configuring CiliaBots through guided, modular tissue aggregation

Ciliated biobots, or CiliaBots, are a class of engineered multicellular tissues that are capable of self-actuated motility propelled by the motile cilia located on their exterior surface. Correlations have been observed between CiliaBot motility patterns and their morphology and cilia distribution. However, precise control of these structural parameters to generate desired motility patterns predictably remains unexplored. Here, we developed a novel Aggregated CiliaBot (AggreBot) platform capable of producing designer motility patterns through spatially controlled aggregation of epithelial spheroids made from human airway cells (referred to as CiliaBot Building Blocks or CBBs), yielding AggreBots with configurable geometry and distribution of active cilia. Guided multi-CBB aggregation led to the production of rod-, triangle-, and diamond-shaped AggreBots, which consistently effected greater motility than traditional single-spheroid CiliaBots. Furthermore, CBBs were found to maintain inte..., , , # AggreBots: configuring CiliaBots through guided, modular tissue aggregation

[https://doi.org/10.5061/dryad.7m0cfxq6d](https://doi.org/10.5061/dryad.7m0cfxq6d)

## Description of the data and file structure

7 .zip files are included in this dataset, containing all of the brightfield images, confocal images, and videos needed to reproduce the data seen in the AggreBot manuscript.

### Files and variables

#### File: Figure_2*_*Dryad*_*Curated.zip

**Description:** The materials within are split into six separate folders.

\"2B Images\" contains the brightfield images taken at 20X magnification from which the data points in Figure 2B were extracted. These images are further subdivided into folders depending on the time point after pairing of spheroids (0, 2, 4, 6, and 24 hours after).

\"2B Analysis\" contains the Excel spreadsheet with the morphological data plotted in Figure 2B. time point of image is listed in the first column, followed by the overall length L and central width W_c, bot...,