mRNA profiling in dss1(I) and dss1(V) mutant lines of Arabidopsis thaliana under oxidative stress (hydrogen peroxide)

The Arabidopsis genome contains two highly homologous DSS1 genes, AtDSS1(I) and AtDSS1(V). This study was designed to identify the functional differences between AtDSS1s, focusing on their potential roles in oxidative stress. We generated single dss1(I) and dss1(V) mutant lines of both Arabidopsis DSS1 genes using CRISPR/Cas9 technology. The sensitivity of homozygous mutant lines with large indels (dss1(I)del25 and dss1(V)ins18) were analyzed to oxidative stress. Plant seedlings were exposed to oxidative stress induced by 10 mM hydrogen peroxide. Transcriptome analysis revealed that the absence of either of DSS1 genes caused alterations in the expression of different genes under stress conditions. In order to carry out RNA sequencing of three different biological samples (wild-type, dss1(I), and dss1(V) (mutants under oxidative stress), 400 ng of the isolated RNA per sample were sent to Novogene Bioinformatics Technology Co. Ltd (Cambridge CB4 0FW, United Kingdom) at room temperature stabilized by DNA/RNA shield buffer (Zymo Research, Irvine, USA). RNA material of each sample was collected from three biologically independent experiments.