Western blotting and quantitative RT-PCR was used to identify siRNAs that impact tyrosinase, MITF, and Melan-A protein levels or impact tyrosinase and MITF mRNA levels in MNT-1 cells (Figure 2 A, B).

NIAID Data Ecosystem50

Effect of the NOD1 agonist iE-DAP on CXCL8 release by HMVEC and HPASM.

炎症调控

细胞药理学实验

HMVEC and HPASM were cultured for 24 hours in 96 well plates with media alone or iE-DAP 10 µg/ml. Normalised results expressed as mean percentage change from control ± SEM; 12 (HMVEC) or 8 (HPASM). Re

NIAID Data Ecosystem20

Additional file 1: of Increased PHGDH expression promotes aberrant melanin accumulation

黑色素生成调控

基因功能验证

Figure S1. Generation of the PHGDHtetO allele. Figure S2. Tissues from mice with long-term exposure to doxycycline diet show variable PHGDH expression. Figure S3. PHGDH expression leads to melanin acc

Figshare2019-07-22 更新50

Hyperosmotic Stress Reduces Melanin Production by Altering Melanosome Formation

黑色素生成调控

细胞应激响应

Many tissues of the human body encounter hyperosmotic stress. The effect of extracellular osmotic changes on melanin production has not yet been elucidated. In this study, we determined that hyperosmo

NIAID Data Ecosystem40

The impact of NTS on B16F10 cells

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