Endometriosis in mice and association with dysbiosis during the acute phase of lesion formation. Endometriosis I

The new formation of these lesions is associated with inflammation, angiogenesis and stem cell recruitment, which may lead to an altered composition of the gut mi-crobiota. To test this hypothesis, we herein induced endometriotic lesions by trans-plantation of uterine tissue fragments from green fluorescent protein (GFP)+ donor mice into the peritoneal cavity of GFP- C57BL/6 wild-type mice. Sham-transplanted animals served as controls. Fecal pellets of the animals were collected 3 days before transplantation as well as on day 7 and 21 after the induction of endometriosis to analyze the composition of the gut microbiota by means of 16S ribosomal-RNA gene sequencing.