TNBC- cisplatin resistance metabolomics study

A cisplatin-resistant TNBC cell line was developed and characterized. An untargeted metabolomics study was performed using LC-MS/MS technology. The study was done with biological and technical replicates. QC pools were run in between to check the instrument variation. Primary data analysis and metabolite identification were done using compound discoverer 3.2 and statistical analysis was performed using metaboanalysis 5.0. The target metabolite validation was also done using Parallel Reaction Monitoring (PRM) based analysis.