Obesity and dyslipidemia are associated with partially reversible modifications to DNA hydroxymethylation in swine adipose-derived mesenchymal stem/stromal cells [Sus scrofa]

Female domestic pigs were fed a 16-week Lean or Obese diet. Mesenchymal stem/stromal cells (MSCs) were harvested from subcutaneous adipose tissue and expanded for 3-4 passages, and 5hmC profiles were examined through hydroxymethylated DNA immunoprecipitation sequencing (hMeDIP-seq) We hypothesized that obesity and cardiovascular risk factors induce functionally-relevant, locus-specific changes in overall exonic coverage of 5hmC in swine adipose-derived MSCs, and evaluated their reversibility using an epigenetic modulator, vitamin-C. Overall design: Lean-MSCs and Obese-MSCs were harvested from swine fed a Lean or Obese diet, respectively (n=3, each), and Obese-MSCs were additionally co-incubated with vitamin-C in-vitro