Richard Allen (University of Hawaii) (2011) CIL:12979, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

An image of a cell actively undergoing endocytosis that develops an extensive layer of vesicles and cisternae composed for the most part of smooth undecorated membrane. The uncoated cisternae are early endosomes to which uncoated vesicles that first arise as coated vesicles from the parasomal sacs fuse with the cisternae to deposit their load of receptor-mediated cargo. Smaller clathrin-coated vesicles bud from the early endosomes and these are either recycled back to the plasma membrane or move deeper into the cell as carrier vesicles after they lose their clathrin coats. TEM taken on 5/21/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 26,250X. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12614) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

本数据集呈现一张细胞在进行内吞作用时，形成的由平滑未修饰膜构成的广泛囊泡和囊腔层的图像。无包膜的囊腔属于早期内体，与最初作为有包膜囊泡从周质囊泡中产生、随后融合于囊腔并释放其受体介导的货物载体的无包膜囊泡相结合。较小的网格蛋白包膜囊泡从早期内体中发芽，这些囊泡要么在失去网格蛋白包膜后回收至质膜，要么作为载体囊泡进一步深入细胞。该透射电子显微镜（TEM）图像由R. Allen于1979年5月21日使用日立HU11A仪器在75kV电压下拍摄。负片放大倍数为26,250倍。该负片被打印到纸上，图像随后被扫描至Photoshop中。此数字化图像可用于定性分析。未经处理的、高分辨率版本的图像（CIL:12614）存档于图书馆，可供进行定量分析。采用标准戊二醛固定，随后进行四氧化锇处理，以酒精脱水，并浸泡于环氧树脂中。微切片厚度约为75纳米。更多详细信息请访问（http://www5.pbrc.hawaii.edu/allen/）。