Immunofluorescent imaging of ISMC- or MS-seeded implant.

After two weeks in vivo, ISMC and MS implants on scaffolds were identified with anti-GFP immunofluorescence. Both ISMC and MS expressed SMA, but ISMC had less immunofluorescence for DES and MHC compared to MS. ISMC also showed reduced S100 immunofluorescence compared to MS. Merged images of SMA (green) and s100 (red) show no co-localization of immunofluorescence in either ISMC or MS. BTUB immunofluorescence was decreased in ISMC implants compared to MS implants. Nuclei were indicated with DAPI. 200x magnification, 100-µm scale bars.