five

Chemotherapy selected 4T1 cells

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NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE216079
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10e6 tumor cells were plated in 10 cm cell cuture dish overnight before treatment. For methotrexate selection, cells were treated with the dosage around IC85 in culture medium. The medium with MTX were replaced in 2nd and 3rd day and then every 2-3 days. The treatment was continued for up to 2 weeks until single colonies os surviving cells were visible. Treated and untreated 4T1 cells, in triplicate.
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2024-01-23
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