Adaptation of cultured adipocytes to cool temperatures

The experiment mapped changes in RNA that occur as adipocytes adapt to cool temperatures (31°C ) in vitro. We performed RNA-Seq on mature adipocytes exposed to 31°C for 0, 1 or 12 days. 0 day refers to the control, where cells were cultured at 37°C only. Mesenchymal stem cells (MSCs) were isolated from C57BL/6J mice. Mature adipocytes were exposed to 31°C for 0, 1 or 12 days. For the cells cold exposed for 12 days, the MSCs were cultured at 37°C prior to and for the first four days of adipogenesis, after which cells were moved to 31°C for 12 days. The cells cold exposed for 1 day were moved to 31°C 15 days after adipocyte differentiation. The cells cold exposed for 0 days were cultured at 37°C consistently. All cells were harvested 16 days after adipocyte differentiation.