Michael Cammer, Dianne Cox (2011) CIL:35128, Mus musculus, macrophage. CIL. Dataset

Primary bone marrow macrophages derived from mouse plated on a coverslip and imaged live. Internal Reflection Microscopy (IRM) shows direct apposition of the cells to coverslip with very little depth of field. Note the light gray uniform background extends under the cells' footprints demonstrating that the cells do not adhere tightly to the substrate. At the center of the image is a large polarized lamellipod of a crawling cell. The small spot below the cell is the tip of glass micropipette delivering CSF (colony stimulating factor) locally and the cell may be responding.

来自小鼠的初级骨髓巨噬细胞在载玻片上培养，并进行活细胞成像。内部反射显微镜（IRM）显示细胞与载玻片的直接附着，且景深极浅。请注意，浅灰色均匀的背景延伸至细胞足迹之下，表明细胞并未紧密附着于基底。图像中心可见一只正在爬行细胞的较大偏振层状伪足。细胞下方的小点为玻璃微吸管尖端，正在局部递送CSF（集落刺激因子），细胞可能正在做出响应。