Mass spectrometry identification of proteins in spots up-regulated in or unique to MCF7 as compared to T47D cell line.

The function description and/or biological process and the subcellular location were from the UniProt database (www.uniprot.org). Spot, spot number; FC, fold change; MW, molecular weight; pI exp, isoelectric point as determined from the 2-D gel experiments; Pep, number of unique peptides; U, unique. The number after the protein name indicated the additional spot in which the protein was found.‡Protein previously reported to be used as a breast cancer biomarker and which were overexpressed in cancerous cells based on data from the literature [9], [10].*Protein used for Q-RT-PCR validation.