Analysis of differential gene expression in the degradation of doxycycline by Chryseobacterium sp. WX1. Analysis of differential gene expression in the degradation of doxycycline by Chryseobacterium sp. WX1

We isolated an efficient doxycycline degrading strain Chryseobacterium sp. WX1. To investigate gene expression patterns during doxycyclinedegradation by strain WX1, we conducted a comparative transcriptomic analysis using cultures of strain WX1 with and without doxycycline addition. The RNA-Seq data revealed that 90.44-96.56% of the reads mapped to the genome of Chryseobacterium sp. WX1 across all samples. Differentially expressed genes (DEGs) analysis (|log2FC| >2; p < 0.01) showed that 693 genes were significantly up-regulated and 592 genes were significantly down-regulated. Overall design: Control groups (WX1_CK): cultures of strain WX1 without doxycycline addition. Experimental groups (WX1_DOX):cultures of strain WX1 with 50 mg/L doxycycline.