Genome wide analysis of Suv4-20h1/2 depleted animal caps [dataset3]

Suv4-20h1/2 are histone methyltransferases that write the H4K20me2 and H4K20me3 marks. We knocked down these enzymes using translation blocking morpholinos in X. tropicalis animal caps and performed RNA-seq in order to investigate the impact of altering H4K20me state on epidermal differentiation. Knocking down Suv4-20h1/2 leads to a strong increase of H4K20me1 in bulk chromatin. To determine whether increased H4K20me1 is responsible for transcriptional changes in suv4-20h KD animal caps, we performed RNA-Seq for a rescue experiment with PHF8, an H4K20me1 demethylase. Overall design: RNA-seq experiment of human Phf8 mRNA injection in Suv4-20H1/2 knock down or control conditions (i.e. control morpholino, LacZ mRNA) in 3 biological replicates.