BioGRID Organism Binary Protein-Protein Interactions

Balanced datasets compiled from BioGRID Release 4.4.198 (May 25, 2021) organism data. Provides new benchmarks containing recent data to avoid discrepancies found in older benchmark datasets. Filtering approach: Only physical interactions with high/low throughput are included with the following detection methods: two-hybrid affinity capture-ms affinity capture-western reconstituted complex affinity capture-luminescence co-crystal structure far western fret protein-peptide co-localization affinity capture-rna co-purification Interaction confidence level: Confidence: ( "c0"): all non-redundant interactions listed by BioGRID have been included. High confidence: ( "c1"): only interactions listed by BioGRID multiple times have been included. Very high confidence ("c2"): only interactions listed by BioGRID with multiple different publication sources have been included. Proteins with >40% sequence identity are removed. Negative interactions were generated by random sampling of proteins in positive interactions such that the negative pairs are not found in the positive interactions.