The Addition of Transcriptomics to the Bead-enabled Accelerated Monophasic Multi-omics Method: A Step Toward Universal Sample Preparation

Omics-based measurements enable the study of biomolecules in a high-throughput fashion, leading to the characterization and quantification of biological systems. Multiomics methods aim to incorporate several omics measurements for a more holistic approach, which is crucial for advancing our understanding of the diversity and redundancy of biological systems. Current multiomics sample preparation methods have achieved proteomics, lipidomics, and metabolomics from individual samples; however, the bioinformatic tools currently available for interpreting data generated from these omics are limited. Alternately, transcriptomics has a wide arsenal of available bioinformatic tools offering intensive sample characterization, but has yet to be incorporated into a unified, multiomics sample preparation technique.Herein we describe the modified Bead-enabled Accelerated Monophasic Multi-omics Method (mBAMM), which incorporates RNA extraction for transcriptomics analysis. mBAMM was shown to enable RNA-seq without compromising the isolation of biomolecules for proteomics, lipidomics, and metabolomics. This methodology greatly improves sample characterization and represents a major innovation toward cohesive insights into biological systems.Here we share the RNA sequencing data after RNA extraction by this mBAMM method and by a commercial extraction kit, so that the data may be compared.