single-cell RNA seq of developing and smoc1/2 ko mouse incisor

We used single-cell sequencing to understand the development of incisor tissues from mice. We utilized the single-cell sequencing platform from SeekGene Biotechnology to study the heterogeneity of mesenchyme in incisors at both embryonic and adult stages of mice, and discovered that Smoc2 is a marker gene for mesenchymal stem cells in mouse incisors. We employed the 10X platform to sequence the incisor tissues of smoc1/2 knockout (ko) and control mice during the embryonic stage to investigate the molecular mechanisms underlying the developmental abnormalities in smoc1/2 ko mice.Our study provides a molecular mechanism explaining how stem cells form during development. Single-cell transcriptomes were obtained from the digestion of the incisor pulp at different stages (E13.5, E14.5, E15.5, E16.5, E18.5 and P56) and group (control and Smoc1/2 ko).本研究采用单细胞测序技术解析小鼠切牙组织的发育过程。借助寻因生物（SeekGene Biotechnology）的单细胞测序平台，我们对小鼠胚胎期与成年期切牙组织的间充质异质性开展研究，发现Smoc2可作为小鼠切牙间充质干细胞的标记基因。为探究Smoc1/2基因敲除（ko）小鼠发育异常的分子机制，本研究采用10X平台，对胚胎期Smoc1/2 ko小鼠与对照小鼠的切牙组织进行测序。本研究阐明了发育过程中干细胞形成的分子机制，通过酶解切牙髓组织，获取了不同发育阶段（E13.5、E14.5、E15.5、E16.5、E18.5及P56）及对照、Smoc1/2 ko两组小鼠的单细胞转录组数据。