Breeding site microbiota is shaped through oviposition by female Aedes aegypti

Aedes aegypti, the main vector of multiple arboviruses, is highly associated with human dwellings. Females exhibit an opportunistic oviposition behavior, seldomly laying eggs on natural containers, but rather distributing them among human-generated breeding sites. Bacterial communities associated with such sites, as well as the compositional shifts they undergo through the development of larval stages, have been described. Some bacteria can play a direct role in supporting the success of mosquito development. Additionally, exposure to different bacteria during larval phases can have an impact on life-history traits. Whether the larvae acquire symbionts from aquatic niches, or just require bacteria as food, is still debated. Here we present an amplicon based sequencing of the 16s rRNA gene, V4 region 515F-806R. The amplicons were sequenced on the Illumina MiSeq platform.