Ectopic misexpression of Irx3 and Irx5 in the paraventricular nucleus of the hypothalamus contributes to hyperphagia and neurodevelopmental defects in Sim1 haploinsufficiency. Ectopic misexpression of Irx3 and Irx5 in the paraventricular nucleus of the hypothalamus contributes to hyperphagia and neurodevelopmental defects in Sim1 haploinsufficiency

Single cell sequencing of Sim1-Cre;tdTomato+ FACS sorted cells from hypothalami of wildtype and Sim1 heterozygous mice. Overall design: Single-cell RNA sequencing (Drop-Seq) was performed on tdTomato+ FACS sorted cells hypohalami of Sim1-Cre;tdTomato and Sim1-Cre;tdTomato;Sim1Het (double-heterozygous) mice at postnatal day 1 to 4. For each genotype, hypothalamus tissues from 6 pups were pooled for single-cell dissociation, followed by FACS sorting and single-cell RNA sequencing.