Transcription profiling of mouse ovaries from newborns

Microarray analysis of normal newborn ovarian transcript levels, for use in comparison to array based studies of differential expression in mouse knockout models. Experiment Overall Design: Newborn ovaries were pooled separately from wild type animals and total RNA isolated using RNeasy mini kit (Qiagen, CA). Newborn ovaries were collected within 12 hours of delivery. Animal experimentation was approved by the Institutional Animal Care and Use Committee of Baylor College of Medicine. Three independently pooled RNA samples from wild type used to generate biotinylated cRNA. Biotinylated cRNA was hybridized to GeneChip Mouse Expression Set 430 2.0 (Affymetrix, Inc.). Since three independent experiments were performed from three independent pools of wild type RNA, signal intensities for particular genes were averaged between the three chips.本数据集为正常新生小鼠卵巢转录本水平的微阵列（Microarray）分析数据，用于与基因敲除（knockout）小鼠模型的差异表达芯片研究开展对比。 实验总体设计如下：于分娩后12小时内收集新生小鼠卵巢，将野生型（wild type）动物的新生卵巢单独混合后，使用RNeasy迷你试剂盒（Qiagen公司，加利福尼亚州）提取总RNA。本动物实验已获得贝勒医学院实验动物管理与使用委员会的批准。制备3份独立的野生型RNA混合样品，用于合成生物素标记的互补RNA（cRNA）。将生物素标记的cRNA与GeneChip小鼠表达谱芯片430 2.0（Affymetrix公司）进行杂交。由于本研究基于3份独立的野生型RNA混合样品开展了3次独立实验，因此对3张芯片上特定基因的信号强度取平均值。