Table 2 in Pre-treatment of canine plasma with heat, rather than acid, efficiently enhances Dirofilaria immitiS antigen detection

Table 2 OD values from twofold serial dilutions of positive plasma sample (control) and four ICD protocols DilutionNo treatmentColorICD-1P ***ColorICD-2PColorICD-3PColorICD-4PColorOD ±SDOD ±SDOD ± SDOD ±SDOD ±SD2 10.435 ±0.066*+ **0.525 ±0.0390.02+0.624 ±0.01710 −4+0.392 ±0.0200.22+0.534 ± 0.0260.01+2 20.385 ±0.063+0.472 ±0.0650.02+0.568 ±0.03310 −4+0.285 ±0.0410.01+0.446 ± 0.0230.07+2 30.279 ±0.021+0.510 ±0.01510 −4+0.513 ±0.00610 −4+0.292 ±0.0050.54+0.377 ± 0.00810 −3+2 40.184 ±0.004+0.436 ±0.00610 −4+0.441 ±0.02710 −4+0.299 ±0.01910 −4+0.250 ± 0.01310 −4+2 50.128 ±0.013+0.318 ±0.01110 −4+0.334 ±0.00210 −4+0.118 ±0.0370.19+0.192 ± 0.01410 −4+2 60.069 ±0.002+0.190 ±0.01510 −4+0.215 ±0.01110 −4+0.080 ±0.0170.07+0.099 ± 0.00210 −4+2 70.031 ±0.003+0.085 ±0.01810 −4+0.121 ±0.00610 −4+0.053 ±0.00610 −3+0.046 ± 0.00810 −2+2 80.011 ±0.005−0.044 ±0.01710 −4+0.042 ±0.00410 −4+0.018 ±0.0040.06−0.020 ± 0.0010.02−2 90.006 ±0.002−0.019 ±0.00610 −3+0.011 ±0.0030.04+0.003 ±0.0020.27−0.006 ± 0.0030.94−2 100.007 ±0.002−0.006 ±0.0050.83−0.007 ±0.0030.98−0.011 ±0.0080.37−0.006 ± 0.0000.73−2 110.004 ±0.002−0.002 ±0.0020.22−0.005 ±0.0030.58−0.002 ±0.0020.14−0.010 ± 0.00110 −2−2 120.006 ±0.002−0.004 ±0.0010.45−0.003 ±0.0030.28−0.003 ±0.0020.37−0.013 ± 0.0030.09− * The optical density values are expressed as mean ±standard deviation (SD) ** The color change based on the DiroCheck is read as + (positive) or – (negative) *** P value was calculated by comparing the OD values between NO Treatment group and ICD-1, ICD-2,ICD-3,or ICD-4