Masses (m/z) used to determine palytoxin type (Top) and corresponding palytoxin detection in samples (Bottom).

(Top) Calculated m/z values for palytoxin, 42-hydroxy-palytoxin, and deoxy-palytoxin, and their various derivatives, used to determine palytoxin type in aquarium store zoanthids using high resolution mass spectrometry.(Bottom) Palytoxin type in highly toxic samples determined from information above. [+++] Major toxin: all ions present [except (M+3H)3+ - see text]. [+] Minor toxin: select ions also present as dominant ion in isotopic cluster [mainly (M+2H-H20)2+ and (M+2H+K)3+]. [−] No listed ions detected as dominant ion in isotopic cluster.