Clostridium botulinum whole genome expression profile after heat shock in continous culture. Clostridium botulinum

Clostridium botulinum ATCC 3502 was grown in continuous culture at 39°C, subjected to heat shock at 45°C, and then continuously grown at 45°C. The goal was to determine the impact of stressful temperature to the whole genome expression profile and identify stress adaptation mechanisms. Overall design: Time series (control,0min,10min,1h,18h,42h, adapt) with two biological replicates and two technical replicates in dye-swaps. Common reference hybridization design.