In the course of a Bacillus subtilis functional genomics project which involved screening for sporulation genes, we identified an open reading frame, yaaT, whose disruptant exhibits a sporulation defe
An A+S− strain (DK8615) designated A+ Tra+ was sporulation proficient and arbitrarily set at a 100%; while a nonmotile strain (A− Tra+; DK8601) did not sporulate. The traA::km mutation (Tra−) was cros