DataSheet_1_Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis.xlsx

Scabies, a human skin infestation caused by the ectoparasitic mite Sarcoptes scabiei var. hominis, affects more than 200 million people globally. The prevailing knowledge of the disease process and host immune response mechanisms is limited. A better understanding of the host-parasite relationship is essential for the identification of novel vaccine and drug targets. Here we aimed to interrogate the transcriptomic profiles of mite-infested human skin biopsies with clinical manifestations of ordinary scabies subjects (“OS”; n = 05) and subjects naive to scabies (“control”; n = 03) using RNASeq data analysis. A combined clustering, network, and pathway mapping approach enabled us to identify key signaling events in the host immune and pro-inflammatory responses to S. scabiei infestation. The clustering patterns showed various differentially expressed genes including inflammatory responses and innate immunity genes (DEFB4A, IL-19, CXCL8, CSF3, SERPINB4, S100A7A, HRNR) and notably upregulation of the JAK-STAT pathway in scabies-infested samples. Mite-infested human skin biopsies (GSE178563) were compared with an ex-vivo porcine infested model (E-MTAB-6433) and human skin equivalents (GSE48459). Marked enrichment of immune response pathways (JAK-STAT signaling, IL-4 and IL-13 pathway, and Toll receptor cascade), chemokine ligands and receptors (CCL17, CCL18, CCL3L1, CCL3L3, CCR7), and cytokines (IL-13 and IL-20) were observed. Additionally, genes known for their role in psoriasis and atopic dermatitis were upregulated, e.g., IL-19. The detailed transcriptomic profile has provided an insight into molecular functions, biological processes, and immunological responses and increased our understanding about transcriptomic regulation of scabies in human.

疥疮，一种由人体皮肤寄生虫疥螨（Sarcoptes scabiei var. hominis）引起的皮肤感染，全球影响超过两亿人口。对于疾病进程和宿主免疫反应机制的了解有限。对宿主-寄生虫关系的深入理解对于新疫苗和药物靶点的识别至关重要。本研究旨在利用RNASeq数据分析，调查具有寻常疥疮症状（OS；n = 05）和对疥疮无免疫力的受试者（对照；n = 03）的螨虫感染皮肤活检的转录组特征。通过结合聚类、网络和通路映射方法，我们能够识别宿主免疫和促炎反应中对S. scabiei感染的关键信号事件。聚类模式显示包括炎症反应和先天免疫基因（DEFB4A、IL-19、CXCL8、CSF3、SERPINB4、S100A7A、HRNR）在内的差异表达基因，以及在疥疮感染样本中JAK-STAT通路显著上调。螨虫感染的皮肤活检（GSE178563）与体外感染的猪模型（E-MTAB-6433）和人类皮肤替代品（GSE48459）进行了比较。观察到免疫反应通路（JAK-STAT信号传导、IL-4和IL-13通路以及Toll受体级联反应）、趋化因子配体和受体（CCL17、CCL18、CCL3L1、CCL3L3、CCR7）以及细胞因子（IL-13和IL-20）的显著富集。此外，已知在银屑病和特应性皮炎中起作用的基因（如IL-19）上调。详细的转录组特征为我们揭示了分子功能、生物过程和免疫反应，并加深了我们对于疥疮在人类中转录组调控机制的理解。