Double-selection of MRC1 and MRC5 crossovers.

KAN-BAR F1 trans-hemizygotes were backcrossed to Col wild type female flowers and progeny were double-selected for kanamycin and glufosinate resistance. The numbers of double-resistant versus sensitive backcross seedlings are listed. The physical distances (megabase, Mb) were calculated between the KAN and BAR insertion sites relative to the TAIR10 Col reference assembly [75]. Genetic distance (cM) is calculated as 100×(2×(NKB/Ntotal)), where NKB is the number of Kan-Bar double-resistant backcross progeny and Ntotal is the total number of backcross progeny screened (Double-Resistant+Sensitive).