Effect of Nop53 depletion on the exosome interactome
收藏NIAID Data Ecosystem2026-03-12 收录
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资源简介：
In order to evaluate how Nop53 modulates the interaction of Rrp6 and Rrp43 with other factors, a coimmunoprecipitation assay was performed both in the presence (-doxicycline) and upon depletion (+ doxicycline) of Nop53 using the condional strain Δnop53/tetOff::GFP-NOP53 carrying the Rrp6-TAP or Rrp43-TAP fusion. As a negative control, the same strain carrying only the TAP tag was employed. For each condition, the elution of biological triplicates was subjected to label free quantitative analysis.
创建时间：
2021-09-08
