Levels of osteoprotegerin (), receptor activator of nuclear factor-kappa B ligand (), and ratio in human osteoarthritis subchondral bone osteoblasts

<b>Copyright information:</b>Taken from "Chondroitin and glucosamine sulfate in combination decrease the pro-resorptive properties of human osteoarthritis subchondral bone osteoblasts: a basic science study"http://arthritis-research.com/content/9/6/R117Arthritis Research &amp; Therapy 2007;9(6):R117-R117.Published online 9 Nov 2007PMCID:PMC2246236. Expression and production of , expression of , and expression ratio of L of cells incubated in the absence or presence of chondroitin sulfate (CS) (200 μg/mL), glucosamine sulfate (GS) (50 or 200 μg/mL), or both (200 μg/mL each) in the absence or presence of vitamin Dat 50 nM. Total RNA was extracted and processed for quantitative polymerase chain reaction (qPCR), and the data are expressed as the mean ± standard error of the mean of arbitrary unit. The release of was determined in the culture medium by a specific enzyme-linked immunosorbent assay. Data are from eight independent experiments. Statistical significance was assessed by paired Student test versus autologous control. Underlined value indicates the statistical difference between control (C, basal conditions) and vitamin D-treated specimens.