Table_7_Expression Profile and Potential Functions of Circulating Long Noncoding RNAs in Acute Ischemic Stroke in the Southern Chinese Han Population.XLSX

Background: Long noncoding RNAs (lncRNAs) have been confirmed to be associated with ischemic stroke (IS); however, their involvement still needs to be extensively explored. Therefore, we aimed to study the expression profile of lncRNAs and the potential roles and mechanisms of lncRNAs in the pathogenesis of acute ischemic stroke (AIS) in the Southern Chinese Han population.Methods: In this study, lncRNA and mRNA expression profiles in AIS were analyzed using high-throughput RNA sequencing (RNA-Seq) and validated using quantitative real-time polymerase chain reaction (qRT-PCR). Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and network analyses were performed to predict the functions and interactions of the aberrantly expressed genes. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic value of lncRNAs in AIS.Results: RNA-Seq analysis showed that 428 lncRNAs and 957 mRNAs were significantly upregulated, while 791 lncRNAs and 4,263 mRNAs were downregulated in patients with AIS when compared with healthy controls. GO enrichment and KEGG pathway analyses of differentially expressed genes showed that the apoptosis, inflammatory, oxidative and calcium signaling pathways were potentially implicated in AIS pathology. The PCR results showed that the selected lncRNA-C14orf64 and lncRNA-AC136007.2 were significantly downregulated in AIS. ROC curve analysis showed that the area under the ROC curve (AUC) values of lncRNA-C14orf64 and lncRNA-AC136007.2 between AIS and healthy controls were 0.74 and 0.94, respectively.Conclusion: This study provides evidence of altered expression of lncRNAs and their potential functions in AIS. Our findings may facilitate pathological mechanistic studies of lncRNAs in AIS and provide potential diagnostic biomarkers and therapeutic targets for AIS.

背景：长非编码RNA（lncRNA）已被证实与缺血性卒中（IS）相关；然而，它们的作用仍需进行广泛的探索。因此，本研究旨在研究lncRNA的表达谱及其在南方汉族急性缺血性卒中（AIS）发病机制中的潜在作用和机制。方法：在本研究中，利用高通量RNA测序（RNA-Seq）分析了AIS中的lncRNA和mRNA表达谱，并使用定量实时聚合酶链反应（qRT-PCR）进行验证。通过基因本体（GO）、京都基因与基因组百科全书（KEGG）通路富集和网络分析，预测了异常表达基因的功能和相互作用。采用受试者工作特征（ROC）曲线分析评估了lncRNA在AIS诊断中的价值。结果：RNA-Seq分析显示，与健康对照组相比，AIS患者中428个lncRNA和957个mRNA表达显著上调，而791个lncRNA和4,263个mRNA表达下调。差异表达基因的GO富集和KEGG通路分析表明，细胞凋亡、炎症、氧化和钙信号通路可能在AIS病理学中发挥作用。PCR结果证实，所选的lncRNA-C14orf64和lncRNA-AC136007.2在AIS患者中表达显著下调。ROC曲线分析显示，lncRNA-C14orf64和lncRNA-AC136007.2在AIS患者与健康对照组之间的曲线下面积（AUC）值分别为0.74和0.94。结论：本研究提供了关于lncRNA表达改变及其在AIS中潜在功能的证据。我们的发现可能有助于对AIS中lncRNA病理机制的深入研究，并为AIS提供潜在的诊断生物标志物和治疗靶点。