Kinetics of pHi recovery in MDCK cells.
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The pHi recovery after a NH4Cl pulse was measured in BCECF-AM loaded MDCK cells in low-FCS/PCM without (–ATO) or with (+ATO) 0.05 µmol/L arsenic trioxide (ATO) dissolved in water in a spectrofluorometer. Cells were exposed to NH4Cl free control without or with hexamethyleneamiloride (HMA, 100 µmol/L) to monitor the intracellular acidification and pHi recovery in the absence (Control) or presence of PD-98059 (30 µmol/L), Gö6976 (10 µmol/L) or Schering 28080 (10 µmol/L)+concanamycin (0.1 µmol/L). *P
创建时间：
2015-12-02
