GNPS-The effect of olive mill solid waste (OMSW) on the diversity of medicinal mushrooms specialized metabolism. Negative ion mode

Two mushrooms' species, Hericium and Pleurotus were grown on a mushroom substrate mixed with different percentage of olive mill solid waste (OMSW). Mushroom fruit body (FB) spent mushroom substrate (SMS) and mushroom substrate alone without mushroom mycelium (MS) were extracted by methanol and injected to LC-MS/MS, using electro spray ionization (ESI) in a negative ion mode. (Experimental details included in the methods and protocol's part). computational metabolomic tools have been used to study the effect of the OMSW on the diversity of the mushrooms specialized metabolism.