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Single-cell RNA-seq of Mesp1+ mesoderm from ES cells

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE124454
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We have developed a protocol to generate hematopoietic and cardiac derivatives in vitro by Mesp1 induction in ES cells. The goal of this study is to analyze the heterogeneity of Mesp1+ mesoderm by single-cell RNA-seq 48 Mesp1-induced single cells were captured using the Fluidigm C1 microfluidic system. RNA extraction, RT and cDNA amplication were then performed according to the manufacturer's manual.
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2019-03-21
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