ICP-MS analysis of zinc content in ZNF261(1-495).
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Recombinant ZNF261(1-495) was dialyzed overnight against assay buffer (25 mM HEPES pH 7.3, 350 mM NaCl, 5 mM β-mercaptoethanol) or assay buffer containing one of the following treatments: 6 M GuHCl, 50 mM EDTA, 10 mM 1,10-phenathroline, or 10 mM 1,7-phenathroline. Zinc content was determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS).
创建时间：
2014-08-18
