We used Escherichia coli transformed with pJKR-H-tetR plasmids encoding an inducible GFP gene as a model system to assess and optimize MGE-FISH on a confocal microscope. We designed FISH probes for th
Benchmarking the performance of FISH-RACS-Seq based on a mock microbial community, consisting of Bacillus subtilis H6, Escherichia coli K-12 DH5a, Micrococcus luteus D11 and Saccharomyces cerevisiae B
Images of bacteria in human dental plaque after fluorescence in situ hybridization, hyperspectral imaging with a Zeiss LSM 780 or LSM 880 laser-scanning confocal microscope, and spectral unmixing. Ea