Transcriptome analysis from wild type and nHOTAIRM1 knock-out iPSC-derived spinal motor neurons

In the central nervous system, motor neurons (MNs) are responsible for controlling essential body functions. Long noncoding RNAs have emerged overwhelmingly as gene expression regulators crucial at any step of MN differentiation and function. To uncover the function of the neuronal lncRNA nHOTAIRM1, abundantly expressed in spinal MNs, we generated, analysed and compared RNA-Seq data from wild type (WT) and nHOTAIRM1 knock-out (KO) MNs derived from iPSCs through a differentiaton protocol of 12 days Overall design: Three poliA+ RNA samples derived from nHOTAIRM1 KO spMNs and 3 control samples from WT spMNs were subjected to RNA-Seq analysis.