Methods for producing genetic modifications in a plant genome without incorporating a selectable transgene marker， and compositions thereof

Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell， without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide / Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant， plant cell or seed， without incorporating a selectable transgene marker. Once a genomic target site is identified， a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide / Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell， without incorporating a selectable transgene marker.