Digitalis lanata

Leaf and root tissues from three different seedlings were used to prepare the Illumina sequence library. Total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen,Germantown, MD, USA). The sequencing library was prepared from total RNA using the TrueSeq Ribo-Zero Plant RNA library prep kit (Illumina, San Diego, CA, USA) that removes ribosomal RNA. A quality check of the library was carried out In an Agilent 2100 bioanalyzer. The library was sequenced using Illumina HiSeq 2500 to generate 100 bp paired-end raw reads.