Expression data from human foreskin fibroblast and human iduced pluripotent stem cell. Expression data from human foreskin fibroblast and human iduced pluripotent stem cell

Pluripotent cells are important and unique because of unprecedently high proliferation and differentiation potentials. The state of pluripotency is robust enough to sustain years of uninterrupted proliferation in vitro, but, once lost, there is a significant barrier for differentiated cells to regain pluripotency. By now, reprogramming of somatic cells into a pluripotent state is unambiguously proven only for methods that necessarily involve ectopic expression or direct delivery of exogenous factors inside the cells. Molecular mechanisms that provide robustness to the state of pluripotency and sustain the barrier between pluripotent and differentiated cells are not fully understood. We reprogrammed human foreskin fibroblasts into human induced pluripotent stem cells using CoMiP 4in1 plasmid without shRNA p53. Overall design: Human foreskin fibroblasts were reprogrammed into human induced pluripotent stem cells and both cell lines were grown until exponential growth phase. We then extracted mRNA for hybridization on Affymetrix microarrays.