Homo sapiens Colon cancer Cell line RNA-Seq. Homo sapiens

A growing body of genomic data on human cancers poses the critical question of how genomic variations translate to cancer phenotypes. We used standardized shotgun proteomics and targeted protein quantitation platforms to analyze a panel of 10 colon cancer cell lines differing by mutations in DNA mismatch repair (MMR) genes. In addition, we performed transcriptome sequencing (RNA-seq) to enable detection of protein sequence variants from the proteomic data. In addition, RNA-seq data also provide transcript expression information, which can be combined with protein expression levels to identify regulatory changes in biologic systems