Data and statistical calculations for protein standard and tubulin IP peptides.

Peptides identified in both MALDI-TOF and LC-MS/MS experiments were subjected multiple replicate measurements on the MALDI-TOF instrument and descriptive statistics were calculated on the population of data for each peptide (12–23 individual measurements from 23 complete spectra, see Materials and Methods). The exact mass for each peptide was calculated in Protein Prospector (UCSF and compared to the masses observed for the Q-TOF LC-MS/MS system (Q-TOF) and the mean of replicate measurements for the MALDI-TOF instrument (MALDI MEAN). The standard deviation (MALDI SD) is reported in amu and the error (Error) was calculated in PPM. The number of replicate measurements (N), the raw Shapiro-Wilk threshold criteria (Shapiro-Wilk (PASS)) and Grubbs Outlier results are reported in their respective columns. Note that Peptide THNLEPYFESFINNLR is listed in line 23 and 24 with all mass spectral measurements (N = 19) for this peptide used for the statistical data in line 24 (Denoted with a *). However, Grubbs Outlier Test analysis indicated positive outliers for two spectral measurements in these data, which were removed (N = 17) to provide the statistical information presented in line 23.Data and statistical calculations for protein standard and tubulin IP peptides.