Siginificance of Saccharomyces cerevisiae Cdc55p on gene expression at initial stage of alcoholic fermentation. Siginificance of Saccharomyces cerevisiae Cdc55p on gene expression at initial stage of alcoholic fermentation

Our previous report revealed that protein phosphatase 2A (PP2A), complexed with the B55delta-type regulatory subunit (i.e. Cdc55p), is solely responsible for the outstanding glycolytic activity of sake yeast strains (Watanabe et al., Appl. Environ. Microbiol. 85, e02083-18 (2019). However, how PP2A mediates yeast alcoholic fermentation remains elusive. Thus, RNA-seq analysis of S. cerevisiae cdc55-delta cells at the initial fermentation stage was performed to identify the downstream effector targeting the glycolytic control. Overall design: Cdc55p-deficient cells (cdc55-delta) and their control (wild type) of S. cerevisiae BY4741 strain at 6 h after inoculation in YPD20 medium were collected and subjected to RNA-seq analysis.