Bouton-Specific Diversity of Glutamate Release from Single Parallel Fiber Axons in the Cerebellum

This dataset contains recording-level fluorescence time series and derived measurements used to investigate bouton-to-bouton diversity in glutamate release and short-term plasticity along cerebellar parallel fibers. It accompanies the study “Bouton-Specific Diversity of Glutamate Release from Single Parallel Fiber Axons in the Cerebellum.” The release comprises 427 uniquely identified recordings representing 347 physical boutons. Sixty-five boutons were recorded under more than one experimental condition. The experiments include 20 Hz and 50 Hz stimulation trains and extracellular calcium conditions of 1.5, 2.5, and 4 mM. Biological annotations—including sex and postsynaptic target classification—are provided where available. Recording-level fluorescence traces are supplied as open CSV files. Consolidated tables provide bouton metadata, trial-level measurements, baseline fluorescence values, null-amplitude distributions, averaged traces, corrected and uncorrected pulse amplitudes, paired-pulse and train-response ratios, failure estimates, and noise thresholds. Supplementary saturation-experiment amplitudes and traces are also included. Each recording is assigned a canonical identifier linking its metadata, raw converted time series, trial measurements, traces, and derived metrics. SCHEMA.md documents the file organization, identifiers, table granularity, and column definitions, while run_settings.json records the analysis configuration and provenance. The deposit contains the converted fluorescence time series and the numerical data needed to reproduce the reported analyses. Original large microscopy TIFF stacks and intermediate diagnostic figures are not included. Analysis software is deposited separately and linked through the related identifier attached to this record.