3'RNA-seq of murine CD142- ASPCs co-cultured with CD142+ ASPCs (Aregs) carrying specific gene knockdowns

To study the involvement of key Areg (CD142+ ASPC)-specifics factors in the inhibitory capacity of CD142+ ASPCs on adipogenesis, we performed transcriptomic profiling of CD142- ASPCs exposed to the secretome of CD142+ ASPCs carrying knockdowns of the indicated genes. CD142- ASPCs were co-cultured and co-differentiated (within the transwell set-up) with CD142+ ASPCs in which siRNA-based knockdowns of specific genes were performed. ASPCs were collected as Lin- (CD31- CD45- TER119-) CD29+ CD34+ SCA1+ cells of the mouse subcutaneous stromal vascular fraction using FACS.