threonine + tRNA(Thr) + ATP => Thr-tRNA(Thr) + AMP + pyrophosphate

TARS2 (mitochondrial threonyl tRNA synthetase) catalyzes the reaction of threonine, mitochondrial tRNA(Thr), and ATP to form Thr-tRNA(Thr), AMP, and pyrophosphate. The TARS2 gene has been identified by computational analysis of the human genome sequence; its function has been inferred from those of the biochemically characterized mitochondrial aspartyl and tyrosyl tRNA synthetases (Bonnefond et al. 2005).

TARS2（线粒体苏氨酸tRNA合成酶）催化苏氨酸、线粒体苏氨酸tRNA（Thr）和ATP的反应，生成Thr-tRNA(Thr)、AMP和焦磷酸。TARS2基因通过分析人类基因组序列的计算机分析而被识别；其功能基于已生化鉴定并表征的线粒体天冬氨酸和酪氨酸tRNA合成酶（Bonnefond等，2005年）的功能推断而来。