Transcriptome profile of livers from hepatocyte-specific Slc25a34 knockout mice on chow and fast-food diet

Identifcation of diferentially expressed genes in livers of mice maintained on chow diet or fast-food diet when Slc25a34 is deleted in hepatocytes. LoxP sites were inserted into the promoter and intron 1 of Slc25a34 genomic DNA to generate a floxed allele. To specifically delete Slc25a34 in hepatocytes from 2-month-old floxed mice, Cre recombinase was delivered by adeno-associated virus driven by the thyroxine binding globulin (TBG) promoter, AAV8-TBG-Cre, which targets >95% of hepatocytes, generating a hepatocyte-specific Slc25a34 knockout (Slc-HKO). As a control, Slc25a34 floxed mice were injected with AAV8-TBG-GFP, which does not affect Slc25a34 expression (WT control). Mice were maintained on chow diet for 1 month after AAV8 injection and then maintained on either chow diet for 2 additional months or FFD for 2 months. RNA was isolated from whole liver after fasting for 16 hours (Slc-HKO, n = 3 / diet; WT control, n = 3 / diet).