E96A TMZ Treatment Study

Temozolomide (TMZ) induces DNA alkylation lesions repaired primarily through base excision repair. To characterize transcriptional responses to TMZ in the context of impaired APE1 endonuclease activity, RNA-seq was performed on Pa03C Cas9 and E96A mutant cell lines following treatment. Cells were treated with TMZ for 4 hours or 8 hours, along with untreated controls. This dataset captures genotype-specific and time-dependent transcriptional responses to alkylation stress in PDAC cells. Overall design: Pa03C PDAC cell lines carrying either a Cas9 control allele or a homozygous APE1 E96A endonuclease-deficient mutation were treated with temozolomide (TMZ) or left untreated. For each genotype, cells were collected at baseline (untreated), 4 hours after TMZ treatment, and 8 hours after TMZ treatment. Total RNA was isolated each individual sample. This design enables comparison of genotype-specific and time-dependent transcriptional responses to TMZ-induced DNA damage