Effects of Continuous Cropping and Crop Rotation on Fungal Diversity in Greenhouse Soil

[Objectives] To explore the effects of continuous cropping and crop rotation on fungal diversity in greenhouse soil. [Methods] Using the greenhouse soil grown with Ganoderma leucocontextum in different continuous (rotation) cropping years as experimental materials, the change trend of soil fungal diversity in different continuous (rotation) cropping years was studied. Through high-throughput sequencing technology combined with related bioinformatics methods, the richness, diversity index and community structure of the fungal ITS1+ITS2 region in the soil were analyzed. [Results] Continuous cropping or crop rotation could increase the diversity of microbial communities. Ascomycota, Basidiomycota and Zygomycota were dominant fungi. The proportion of Basidiomycota gradually increased with the increase of continuous cropping years, and the abundance decreased after crop rotation. [Conclusions] This study is intended to provide a theoretical basis for the research on continuous cropping and crop rotation obstacles.