FtsK is critical for the assembly of the unique divisome complex of the FtsZ-less Chlamydia trachomatis: blots

# FtsK is critical for the assembly of the unique divisome complex of the
FtsZ-less *Chlamydia trachomatis*: blots
[https://doi.org/10.5061/dryad.1ns1rn94w](https://doi.org/10.5061/dryad.1ns1rn94w) ## Description of the data and file structure **Figure 1, figure supplement 1A-Source data 1**: Unlabeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE). Lysates were prepared from uninfected HeLa cells and HeLa cells infected with *Ct* L2. At 21 hpi, lysates were prepared and characterized by immunoblotting with FtsK-specific antibodies.  **Figure 1, figure supplement 1A-Source data 2**: Labeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE). Lysates were prepared from uninfected HeLa cells and HeLa cells infected with *Ct* L2. At 21 hpi, lysates were prepared and characterized by immunoblotting with FtsK-specific antibodies. **Figure 1, figure supplement 1B-Source data 3**: Unlabeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE). HeLa cells were infected with *Ct* transformed with FtsK-mCherry (molecular mass - 114,664 Da). The fusion was induced with 10nM aTc at 17 hpi. HeLa cells were harvested at 21 hpi, and a lysate was prepared and characterized by immunoblotting analysis with a rabbit polyclonal mCherry antibody. **Figure 1, figure supplement 1B-Source data 4**: Labeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE). HeLa cells were infected with *Ct* transformed with FtsK-mCherry (molecular mass - 114,664 Da). The fusion was induced with 10nM aTc at 17 hpi. HeLa cells were harvested at 21 hpi, and a lysate was prepared and characterized by immunoblotting analysis with a rabbit polyclonal mCherry antibody. **Figure 2, figure supplement 1A-Source data 1**: Unlabeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE).  HeLa cells were infected with *Ct* transformed with mCherry-PBP2 (molecular mass - 150,842 Da), or mCherry-PBP3 (molecular mass - 100,150 Da). The fusions were induced with 10nM aTc at 17 hpi. HeLa cells were harvested at 21 hpi, and lysates were prepared and characterized by immunoblotting analysis with a rabbit polyclonal mCherry antibody. **Figure 2, figure supplement 1A-Source data 2**: Labeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE).  HeLa cells were infected with *Ct* transformed with mCherry-PBP2 (molecular mass - 150,842 Da), or mCherry-PBP3 (molecular mass - 100,150 Da). The fusions were induced with 10nM aTc at 17 hpi. HeLa cells were harvested at 21 hpi, and lysates were prepared and characterized by immunoblotting analysis with a rabbit polyclonal mCherry antibody. **Figure 2, figure supplement 1A-Source data 3**: Unlabeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE).  HeLa cells were infected with *Ct* transformed with mCherry-MreC (molecular mass - 63,906 Da). The fusion was induced with 10nM aTc at 17 hpi. HeLa cells were harvested at 21 hpi, and lysates were prepared and characterized by immunoblotting analysis with a rabbit polyclonal mCherry antibody. **Figure 2, figure supplement 1A-Source data 4**: Labeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE).  HeLa cells were infected with *Ct* transformed with mCherry-MreC (molecular mass - 63,906 Da). The fusion was induced with 10nM aTc at 17 hpi. HeLa cells were harvested at 21 hpi, and lysates were prepared and characterized by immunoblotting analysis with a rabbit polyclonal mCherry antibody. **Figure 2, figure supplement 2A-Source data 1**: Unlabeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE).  HeLa cells were infected with *Ct* transformed with mCherry-PBP2. The fusion was induced (+aTc) at 17 hpi or was uninduced. The induced and uninduced cells were harvested at 21 hpi, and lysates were prepared and characterized by immunoblotting analysis with rabbit antibodies raised against peptides derived from chlamydial PBP2. The PBP2 antibody primarily detects a species of ~120kD in the induced sample.  **Figure 2, figure supplement 2A-Source data 2**: Labeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE).  HeLa cells were infected with *Ct* transformed with mCherry-PBP2. The fusion was induced (+aTc) at 17 hpi or was uninduced. The induced and uninduced cells were harvested at 21 hpi, and lysates were prepared and characterized by immunoblotting analysis with rabbit antibodies raised against peptides derived from chlamydial PBP2. The PBP2 antibody primarily detects a species of ~120kD in the induced sample. **Figure 2, figure supplement 2A-Source data 3**: Unlabeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE).  HeLa cells were infected with *Ct* transformed with mCherry-PBP3. The fusion was induced (+aTc) at 17 hpi or was uninduced. The induced and uninduced cells were harvested at 21 hpi, and lysates were prepared and characterized by immunoblotting analysis with rabbit antibodies raised against peptides derived from chlamydial PBP3. The PBP3 antibody primarily detects a single species with the predicted molecular mass of mCherry-PBP3 in the induced sample. **Figure 2, figure supplement 2A-Source data 4**: Labeled, uncropped Western Blot .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE).  HeLa cells were infected with Ct transformed with mCherry-PBP3. The fusion was induced (+aTc) at 17 hpi or was uninduced. The induced and uninduced cells were harvested at 21 hpi, and lysates were prepared and characterized by immunoblotting analysis with rabbit antibodies raised against peptides derived from chlamydial PBP3. The PBP3 antibody primarily detects a single species with the predicted molecular mass of mCherry-PBP3 in the induced sample. ## Code/software .zip file obtained from LICOR Odyssey imaging system (LICOR, Lincoln, NE)