Transcriptomic analysis to elucidate the response of Apis mellifera ligustica brain tissue to fluvalinate exposure

As a commonly used acaricide in apiculture, fluvalinate is used to kill Varroa mites, while it also damages the nervous system of honeybees. To date, the transcriptomic characteristics associated with fluvalinate-induced neuronal injury in the bee brain have not been reported. Here, we performed transcriptome sequencing on Apis mellifera ligustica (A. mellifera ligustica) brain tissues collected before and after fluvalinate treatment. A total of 546 differentially expressed genes (DEGs) were detected, and these DEGs mainly showed 4 different expression patterns. Further analysis revealed that DEGs with different expression patterns were mainly involved in lipid metabolism, amino acid metabolism, visual transduction, and neural response-related GO terms and KEGG pathways. Moreover, protein–protein interaction network analysis revealed five protein-coding DEGs as key genes, which may play important roles in the resistance to fluvalinate-induced honeybee brain nerve tissue damage. In summary, this study is the first to perform a detailed characterization and functional analysis of genes related to fluvalinate stimulation in honeybee brains.